Detection of Staphylococcal Enterotoxin in Food

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Detection of Staphylococcal Enterotoxin in Food.

Methods are described for the extraction and serological detection of trace amounts of enterotoxins A and B in foods incriminated in outbreaks of staphylococcal food poisoning. Evidence is presented for the probable applicability of the methods for the detection of unidentified enterotoxins.

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Outbreak of staphylococcal enterotoxin food poisoning.

Coagulase positive staphylococci are generally difficult to grow in foodstuffs without substantial temperature abuse and foodborne outbreaks are uncommon. The following incident resulted in the first detection of staphylococcal enterotoxin in food in a Queensland outbreak and is the first reported outbreak of staphylococcal foodborne illness in Queensland since 1997 when 42 people in a Bundaber...

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Basis of Virulence in Enterotoxin-Mediated Staphylococcal Food Poisoning

The Staphylococcus aureus enterotoxins are a superfamily of secreted virulence factors that share structural and functional similarities and possess potent superantigenic activity causing disruptions in adaptive immunity. The enterotoxins can be separated into two groups; the classical (SEA-SEE) and the newer (SEG-SElY and counting) enterotoxin groups. Many members from both these groups contri...

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Biosensor detection of botulinum toxoid A and staphylococcal enterotoxin B in food.

Immunoassays were developed for the simultaneous detection of staphylococcal enterotoxin B and botulinum toxoid A in buffer, with limits of detection of 0.1 ng/ml and 20 ng/ml, respectively. The toxins were also spiked and measured in a variety of food samples, including canned tomatoes, sweet corn, green beans, mushrooms, and tuna.

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Detection of the staphylococcal enterotoxin D-like gene from staphylococcal food poisoning isolates over the last two decades in Tokyo

The plasmid is a very well-known mobile genetic element that participates in the acquisition of virulence genes, such as staphylococcal enterotoxins (SEs), via horizontal transfer. SEs are emetic toxins and causative agents in staphylococcal food poisoning (SFP). We herein identified the types of plasmids harbored by seven SFP isolates and examined their production of plasmid-related SE/SEl to ...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1965

ISSN: 0003-6919

DOI: 10.1128/am.13.2.181-189.1965